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ORIGINAL ARTICLE
Year : 2017  |  Volume : 34  |  Issue : 2  |  Page : 88-92

Evaluation of nanogold-beads-based enzyme-linked immunosorbent assay for detection of cryptosporidium antigen in stool samples of diarrheic patients


Parasitology Department, Benha University, Benha, Egypt

Correspondence Address:
Rabab E.O El Attar
45 Arafa Street, Macheit El Nour, Behna, Qalyubia Governorate, 13511
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/bmfj.bmfj_109_17

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Background Biomedical nanotechnology is providing opportunities for rapid and simple diagnosis of many infectious diseases. Aim The aim of this study was to evaluate nanogold-beads-based enzyme-linked immunosorbent assay (ELISA) for the detection of Cryptosporidium antigen in stool samples of diarrheic patients. Patients and methods Two hundred stool samples were collected from diarrheic patients attending Abo El-Resh Hospital. Parasitological examination of the collected samples was performed by direct smear, concentration method, and staining technique. Immunological examination was performed by sandwich ELISA and nanosandwich ELISA. Results There were five (2.5%) stool samples that were positive for Cryptosporidium spp. detected by merthiolate–iodine–formaldehyde concentration technique. There were 47 (23.5%) stool samples positive for Cryptosporidium spp. detected by modified Ziehl–Neelsen staining. There were 78 (39%) stool samples positive for Cryptosporidium spp. detected by sandwich ELISA and 89 (44%) stool samples positive for Cryptosporidium spp. detected by nanosandwich ELISA. The sensitivity of sandwich ELISA and nano-ELISA for the detection of Cryptosporidium antigen in human stool samples was 68 and 85%, respectively. The specificity of sandwich ELISA was 77.3%, but the specificity of nano-ELISA was 73.4%. Conclusion The nanosandwich ELISA improves the sensitivity and specificity of sandwich ELISA.


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