Year : 2018 | Volume
: 35 | Issue : 3 | Page : 419--428
Evaluation of modified semisolid Rappaport Vassiliadis medium in comparison with conventional media in the isolation of Salmonella species from different samples
Soheir Abd El Rahman Abd El Samie1, Yasser Mahmoud Ismail1, Sahar Mohammed Fayed1, Shaimaa Sobhy Hamed2
1 Department of Clinical and Chemical Pathology, Benha University, Benha, Egypt
2 Department of Medicine and Surgery, Benha University, Benha, Egypt
Objectives The goal of this study is to assess the performance of the modified semisolid Rappaport Vassiliadis media (MSRV) for detecting Salmonella spp. in comparison with conventional media especially xylose lysine desoxycholate agar (XLD agar) and Salmonella Shigella (SS) agar.
Results The analysis showed that the overall Salmonella prevalence was 10% (18/180). Of the 18 Salmonella-positive samples, 16 (89%) were isolated from patients suffering from gastroenteritis, while one Salmonella strain (5.5%) was detected in the 45 stool samples of food handlers, and one Salmonella strain (5.5%) isolated from the 45 food samples. SS culture results comparable to serotyping resulted in sensitivity, specificity, positive predictive, negative predictive, accuracy values of 83.3, 98.2, 83.3, 98.2, and 96.7%, respectively, while XLD generated 83.3% sensitivity, 97.5% specificity, 79% positive predictive value, 98.1% negative predictive value, 96.1% accuracy. Statistically, the MSRV method resulted in higher values of sensitivity (94.4%), specificity (99.4%), positive predictive value (94.4%), and negative predictive value (99.4%), and accuracy (98.9%) than those obtained by the other two culture methods.
Conclusions The current study showed that the overall diagnostic performance of MSRV for the detection of Salmonella spp., including sensitivity, specificity, and accuracy, was significantly (P<0.05) greater than that of SS and XLD culturing methods. The incidence of Salmonella in diarrheal patients was significantly (P<0.05) higher than in food handlers and food samples. The occurrence of Salmonella was not significantly (P<0.05) related to the age and the sex of diarrheal patients. Salmonella typhimurium and Salmonella lamberhurst were the most prevalent serotypes in the tested samples.
Dr. Shaimaa Sobhy Hamed
|How to cite this article:|
Abd El Samie SA, Ismail YM, Fayed SM, Hamed SS. Evaluation of modified semisolid Rappaport Vassiliadis medium in comparison with conventional media in the isolation of Salmonella species from different samples.Benha Med J 2018;35:419-428
|How to cite this URL:|
Abd El Samie SA, Ismail YM, Fayed SM, Hamed SS. Evaluation of modified semisolid Rappaport Vassiliadis medium in comparison with conventional media in the isolation of Salmonella species from different samples. Benha Med J [serial online] 2018 [cited 2020 Sep 20 ];35:419-428
Available from: http://www.bmfj.eg.net/article.asp?issn=1110-208X;year=2018;volume=35;issue=3;spage=419;epage=428;aulast=Abd;type=0